Finding the Principle Leads using GC-MS and Unravelling the Anti-inflammatory Activity of Alkaloid Isolated from Caesalpinia bonducella by in vitro Techniques

Abstract: Aim/Background: Plant-based medicines have gained remarkable recognition recently, as consumers begun to express their preference for herbal remedies. C. bonducella, commonly known fever nut, is said massive value in Ayurveda regarding health and cosmetics. We aim envisage the alkaloid its anti-inflammatory property using in-vitro techniques. Materials Methods: bonducella powder, soxhlet apparatus, solvents including hexane, DCM, ethyl acetate methanol. GC-MS, L929 cells, DMEM medium, antibiotic solution, sulfanilamide, naphthylethylenediamine, phosphoric acid, ferric chloride, sodium hydroxide, potassium iodide ammonium hydroxide. Results: The cell-free assay showed 75% Membrane Stabilization, 125% Albumin denaturation 106% Proteinase inhibition at 1mg/mL of aqueous extract. cell viability remained unaltered even highest tested concentration, with a dose-dependent nitrite count recorded. Conclusion: Alkaloids, carbohydrates flavonoids presence solution seed powder. This study that isolated alkaloids found be higher concentration Aqueous extract subsequently employs GC-MS understand various compounds present it. attenuation pro-inflammatory mediator Nitric oxide was significant which supports potential Keywords: Caesalpinia Fever nut seeds, Anti-inflammatory, Alkaloid.